NHP Reagent Resource

Macaque IgG Isotype Reagents - NIH Nonhuman Primate Reagent Resource

BACKGROUND

Rhesus macaques express four IgG isotypes (IgG1, IgG2, IgG3, IgG4) and these have been well characterized (Scinicariello, et al. Immunology 2004; 111:66).

Much of the variation between IgG isotypes occurs within the hinge region, although isotypic variation in amino acid sequences also appears in CH1, CH2 and CH3 domains.

 

In addition to isotypic variation, alleleic polymorphism (or allotypic variation) also occurs between individual athough the exact number of allotypes has not been determined for rhesus macaques.

 

Many monoclonal antibodies capable of distinguishing between human IgG isotypes will react with rhesus IgG. However, most of the anti-human IgG monoclonal antibodies do NOT distinguish between rhesus IgG istoypes.

REAGENTS AVAILABLE

We have expressed recombinant forms of all rhesus IgG isotypes and recombinant antibodies expressing either rhesus kappa or lambda light chains. These are now available as immunoglobulin reference reagents.

COMING SOON

We are developing monoclonal antibodies to distinguish between the four rhesus IgG isotypes.

We are defining the polymorphism within each isotype to describe the allotypic forms of each isotype that can be expressed.